Evaluating The Transcriptional Regulation of Cdh1 by Grhl3 in Different Cellular Models

The calcium-dependent adhesion protein E-cadherin encoded by the Cdh1 gene is a member of large family conferring proper establishment adherens junction. expression critical and observed in epithelial cells. also essential for establishing pluripotent state embryonic stem transcriptional regulation has been focus research many years; early reports identified repressors since its downregulation initiation to mesenchymal transition. Restoring thought be simply due disengagement silencers from promoter. Recent studies supported presence dedicated activators expression, including members Grhl Ets transcription factors. Here we evaluated regulatory potential Grhl3 on promoter different cellular models understand extent relationship with Cdh1. We utilized several approaches, such as correlation loss gain function, ChIP, luciferase reporter assays. As result, found that potent regulator cells origin. Moreover, was sufficient re-establish murine hepatoma Hepa1-6. Improved understanding perception how transitions are regulated, they play crucial role metastasis, which will pave way better management metastatic disease.